3D Spheroids as an In Vitro Model for Fibroblast/CAF Differentiation Using Different TGF-β1 Inhibiting Compounds of Cell-matrix Interaction
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Immunofluorescent Staining of Spheroids
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Results: A 3D Spheroid Model for Cancer-Associated Fibroblasts Differentiation and Invasion
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Conclusion
Trascrizione
Culturing fibroblasts in spheroid recapitulate tumor tissue much better than the commonly used 2D system on stiff plastic. It allows to study fibroblasts differentiation in a tumor-like environment. In this method, the rigidity of cell culture pla
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The goal of this protocol is to establish a 3D in vitro model to study the differentiation of cancer-associated fibroblasts (CAFs) in a tumor bulk-like environment, which can be addressed in different analysis systems, such as immunofluorescence, transcriptional analysis and life cell imaging.