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Expression, Purification, and Liposome Binding of Budding Yeast SNX-BAR Heterodimers

7.6K Views

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10:28 min

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December 6th, 2019

DOI :

10.3791/60413-v

December 6th, 2019

•
7,568 Views
1Department of Cell Biology, Yale School of Medicine, 2Department of Biological Sciences, University of North Carolina at Charlotte

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Keywords SNX BAR
Membrane Remodeling
Liposome Binding
Yeast Expression
Protein Purification
IgG Sepharose Beads
Cell Lysis
Centrifugation
Extrusion
Biophysical Properties
Lipid Specificity
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