In Vitro Differentiation of L. amazonensis Promastigotes into Axenic Amastigotes
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C57BL/6 Footpad Infection with L. amazonensis
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Mouse Footpad Lesion Extraction and Parasite Load Determination
4:29
Results: LIR1 Knockout In Vivo Lesion Development and Intracellular Replication
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Conclusion
Trascrizione
This protocol is suitable for studying Leishmania virulence, because it provides a systemic view of the vertebrate host to the cutaneous infection. The main advantage of adopting this protocol is that it allows researchers to evaluate the host inf
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Here, we present a compiled protocol to evaluate the cutaneous infection of mice with Leishmania amazonensis. This is a reliable method for studying parasite virulence, allowing a systemic view of the vertebrate host response to the infection.