Combined Use of Tail Vein Metastasis Assays and Real-Time In Vivo Imaging to Quantify Breast Cancer Metastatic Colonization and Burden in the Lungs

0:32

Generation of Cancer Cells Stably Expressing Luciferase and a Fluorescent Protein

1:59

Tail Vein Injection of Labeled Cancer Cells

4:17

Monitoring the Metastatic Burden by Bioluminescence with an in vivo Live Animal Imaging Device

6:18

Quantification of the Number and Size of Metastases and Analysis of the Imaging Data

8:01

Results: YAP and TAZ are Required for Metastatic Colonization and Subsequent Growth of Breast Cancer Cells in Syngeneic Mice

9:36

Conclusion