PDX (Patient-Derived Xenografts) Dissociation and Initial Purification of Stromal Component
3:17
Density Gradient Centrifugation-Based Separation of PDX-Derived Clusters from Single Cells
6:03
Hydrogel Preparation and Microfluidic Plate Seeding
10:12
Results: Cell Staining and Viability Assessment
11:16
Conclusion
Trascrizione
Improved culture methods for patient-derived tumor samples are critically important as the field moves away from the use of conventional cell lines. This protocol allows for the in vitro culture of patient-derived tumor cells in a platform that is
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This protocol demonstrates methods to enable extended in vitro culture of patient-derived xenografts (PDX). One step enhances overall viability of multicellular cluster cultures in 3D hydrogels, through straightforward removal of non-viable single cells. A secondary step demonstrates best practices for PDX culture in a perfused microfluidic platform.