Injection and Heat Stress to Promote Rod Formation
4:45
Imaging of Actin Rods with Confocal Microscopy
6:32
Results: Actin Rods in Heat-stressed Embryos
7:17
Conclusion
Trascrizione
Our protocol is significant because it uses the well-established technique of microinjection in Drosophila embryos to allow new research on the actin stress response and accompanying actin rod assembly. The main advantage of this technique is that
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The goal of this protocol is to inject Rhodamine-conjugated globular actin into Drosophila embryos and image intranuclear actin rod assembly following heat stress.