Ovarian Cancer Stem Cell (OCSC) Isolation and Culture
2:23
CSC Passage
3:24
HUEhT-1 Endothelial Cell Culture
4:14
NICO-1 Coculture Plate Preparation
5:16
NICO-1 System HUEhT-1 and CSC Seeding
6:14
Results: Representative CSC-Induced Neovascularization and Vascular Formation
7:19
Conclusion
Trascrizione
Ovarian cancer has a high mortality rate among gynecological malignancies. Ovarian cancer stem cells and their vascular niche are able to remodel tumor microenvironment. However, previous ovarian cancer stem cell models have not permitted the in-d
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Ovarian cancer stem cells (OCSC) are responsible for cancer initiation, recurrence, therapeutic resistance, and metastasis. The OCSC vascular niche is considered to promote self-renewal of OCSCs, leading to chemoresistance. This protocol provides the basis for establishing a reproducible OCSC vascular niche model in vitro.