3-D zebrafish tail explants let's us exploit transparency of zebrafish embryos to its full extent during somite development and vertebrate segmentation. Fill an empty mating tank with fish system water. Clean the strainer with system water.
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Here, we present the protocol for 3-D tissue culture of the zebrafish posterior body axis, enabling live study of vertebrate segmentation. This explant model provides control over axis elongation, alteration of morphogen sources, and subcellular resolution tissue-level live imaging.