KCl-mediated Depolarization of Isolated Synaptic Terminals
3:21
Fixation and Phalloidin Staining of Samples
4:40
Fluorometric Analysis and Light Scattering
5:20
Data Analysis
6:19
Conclusion
Trascrizione
Actin is the major component of cytoskeleton and critically regulates several aspects of cellular morphology and physiology, including in neurons. Actin occurs in equilibrium between its two forms, monomeric globular G-actin or polymeric filamento
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We report a simple, time-efficient and high-throughput fluorescence spectroscopy-based assay for the quantification of actin filaments in ex vivo biological samples from brain tissues of rodents and human subjects.