Making a Standard Inoculation Box with a Removable Top Lid Mounted with a Mesh
1:17
Making an Inoculation Chamber and Inoculating Plants in Flats
3:13
Inoculate Plants with Smaller Inoculation Boxes
3:42
Inoculate Detached Leaves in Petri Dishes
4:37
Results: Assessment of Inoculation Evenness
5:19
Conclusion
Trascrizione
Powdery mildew is an important plant disease. Accurate determination of the disease phenotype requires even inoculation of the pathogen. Since existing methods are inefficient, we have developed this protocol.
Our spore inoculation method ca
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We present a protocol for constructing a simple spore-distribution system consisting of an inoculation box with a ~50 µm mesh and a transparent plastic chamber. This can be used to evenly inoculate plants with powdery mildew spores, thereby enabling accurate and reproducible assessment of disease phenotypes of plants under study.