3D Sprouting Angiogenesis Assay and Immunofluorescence Staining
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Results: Microscopic Analysis and Quantification of Mouse EBs Undergoing Angiogenesis Assay
7:29
Conclusion
Trascrizione
This protocol describes an in vitro sprouting angiogenesis assay that recapitulates many features of the formation of blood vessels and can be used for testing drugs. This method is easy to implement in a lab, robust, and scalable. It has many sim
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This assay utilizes mouse embryonic stem cells differentiated into embryoid bodies cultured in 3D-collagen gel to analyze the biological processes that control sprouting angiogenesis in vitro. The technique can be applied for testing drugs, modeling diseases, and for studying specific genes in the context of deletions that are embryonically lethal.