Results: Alternative Splicing and Polyadenylation in RNA-Seq Data
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Conclusion
Trascrizione
This protocol provides a comprehensive understanding of gene isoforms generated by alternative splicing and polyadenylation by providing a step-by-step workflow to identify differential splicing sites, differentially-expressed exons, and poly(A)si
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Alternative splicing (AS) and alternative polyadenylation (APA) expand the diversity of transcript isoforms and their products. Here, we describe bioinformatic protocols to analyze bulk RNA-seq and 3' end sequencing assays to detect and visualize AS and APA varying across experimental conditions.