The zebrafish is unique in its intrinsic ability to regenerate many different tissue types, including the RPE. This protocol can be used to identify molecular pathways that drive RPE regeneration and RPE disease-related mechanisms. Versatility is
Accedi o inizia la tua prova gratuita per vedere questo contenuto
This protocol describes the methodology to genetically ablate the retinal pigment epithelium (RPE) using a transgenic zebrafish model. Adapting the protocol to incorporate signaling pathway modulation using pharmacological compounds is extensively detailed. A MATLAB platform for quantifying RPE regeneration based on pigmentation was developed and is presented and discussed.