Isolation of Peripheral Blood Mononuclear Cells (PBMCs)
2:49
Expansion of Osteoclasts Progenitors and Induction of Osteoclastogenesis
4:19
Fluorescence Staining of Osteoclasts and Calcium Phosphate Coating
5:17
Results: Analysis of Mature Osteoclast Cultured on Calcium Phosphate Coated Wells
6:38
Conclusion
Trascrizione
Our protocol describes a simple and reliable method for an osteoclastic resorption assay. The use of calcium phosphate coated cell culture plates can be easily prepared and visualized using lab available materials. In contrast to opaque bone or de
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Here, we present a simple and effective assay procedure for resorption pit assays using calcium phosphate coated cell culture plates.