The Peel-Blot Technique: A Cryo-EM Sample Preparation Method to Separate Single Layers From Multi-Layered or Concentrated Biological Samples

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Preparation Steps for the Peel‐Blot Technique

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Placing the Carbon Film onto the Grid

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Separation of Multi-Layered 2D Crystals into Single Layer

4:55

Results: 2D Crystals of Human Leukotriene C4 Synthase and Liposomes Subjected to the Peel‐Blot Method, the Peel-Blot Footprint, and Blotting an EM Grid Prepared by Back-Injection on a Submicron Filter Paper

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Conclusion