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Quantitative Determination of De Novo Fatty Acid Synthesis in Brown Adipose Tissue Using Deuterium Oxide

725 Views

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07:34 min

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May 12th, 2023

DOI :

10.3791/64219-v

May 12th, 2023

•
725 Views
1UCD Conway Institute and UCD Institute of Food and Health, School of Agriculture and Food Science, University College Dublin, 2Division of Endocrinology, Cincinnati Children’s Hospital Medical Center, Cincinnati, 3Division of Developmental Biology, Cincinnati Children’s Hospital Medical Center, Cincinnati, 4Department of Pediatrics, University of Cincinnati College of Medicine

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Keywords Fatty Acid Synthesis
Brown Adipose Tissue
Deuterium Oxide
Non invasive
Isotope labeled Glucose
Metabolic Status
Tissue Dissection
Tissue Extraction
Chloroform methanol Extraction
Fatty Acid Methylation
GC MS Analysis
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