Antibody Staining of FMOs and Single-Color Controls
3:53
Cell Resuspension
4:38
Sample Preparation for Flow Cytometric Analysis
5:19
Gating Strategy for Setting up the Sorting Gates
6:49
Results: Effect of Erythropoietin on Bone Marrow and Spleen Progenitors
7:42
Conclusion
Trascrizione
This protocol allows for the identification and isolation of BFU-E and CFU-E erythroid progenitors directly from freshly-harvested mouse tissue like bone marrow and spleen. Using this technique, we can isolate pure populations of erythroid progeni
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Here, we describe a novel flow cytometric method for prospective isolation of early burst-forming unit erythroid (BFU-e) and colony-forming unit erythroid (CFU-e) progenitors directly from fresh mouse bone marrow and spleen. This protocol, developed based on single-cell transcriptomic data, is the first to isolate all the tissue's erythroid progenitors with high purity.