Real-Time Quantitative PCR (qPCR) Test, Additional PCR Amplification, and Second DNA Purification
6:14
DNA Fragment Size Selection
8:01
Results: Assay for Transposase-Accessible Chromatin with High-Throughput Sequencing
10:19
Conclusion
Trascrizione
ATAC-seq is a powerful technique to understand gene expression regulatory mechanisms. However, adipose tissue is difficult using this technique because of its large lipid contents, high mitochondrial contamination, and cellular heterogeneity. In t
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We present a protocol for assay for transposase-accessible chromatin with high-throughput sequencing (ATAC-seq) specifically on adipocytes using nucleus sorting with adipose tissues isolated from transgenic reporter mice with nuclear fluorescence labeling.