Enrichment of Mycobacterium smegmatis EVs by Density Gradient Centrifugation
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Results: Verifying Enrichment of MsmEVs Containing the EsxA Protein of M. tuberculosis
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Conclusion
Trascrizione
Enriching mycobacterial EVs helps delineate their contents, decipher their factions, and dates designing ways to construct and generate recombinant EVs that mainly exploit vaccine candidates. Six series generate from different locations of the bac
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This protocol details the enrichment of native mycobacterial extracellular vesicles (mEVs) from axenic cultures of Mycobacterium smegmatis (Msm) and how mCherry (a red fluorescent reporter)-containing recombinant MsmEVs can be designed and enriched. Lastly, it verifies the novel approach with the enrichment of MsmEVs containing the EsxA protein of Mycobacterium tuberculosis.