This work was funded by the Key R&#38;D projects of the Science and Technology Department of Sichuan Province (2020YFS0539).

The procedures for the care and use of animals were approved by the Ethics Committee of Mianyang Normal University, and all relevant institutional and governmental regulations concerning the ethical use of animals were strictly followed. For the present study, SPF rats (Kunming species, both male and female, weighing 180-220 g) were utilized. The animals were obtained from a commercial source (see Table of Materials). All animals were housed in a pathogen-free environment and provided ad libitum...

&lt;i&gt;Desmodium caudatum &lt;/i&gt; Decoction&#039;s Impact on Intestinal Microbiota of Rats

<ol>
	<li>Marginean, C. M., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=The+importance+of+accurate+early+diagnosis+and+eradication+in+Helicobacter+pylori+infection:+pictorial+summary+review+in+children+and+adults.">The importance of accurate early diagnosis and eradication in Helicobacter pylori infection: pictorial summary review in children and adults.</a> Antibiotics (Basel). 12 (1), 60 (2022).</li><li>Sipponen, P., Maaroos, H. I. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Chronic+gastritis.">Chronic gastritis.</a> Scandinavian Journal of Gastroenterology. 50 (6), 657-667 (2015).</li><li>Wang, D. J. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Methodological+quality+and+reporting+quality+evaluation+of+chinese+medicine+diagnosis+and+treatment+guidelines+for+chronic+gastritis+in+China.">Methodological quality and reporting quality evaluation of chinese medicine diagnosis and treatment guidelines for chronic gastritis in China.</a> Modernization of Traditional Chinese Medicine and Materia Medica-World Science and Technology. 24 (7), 2776-2783 (2022).</li><li>Burkitt, M. D., Varro, A., Pritchard, D. M. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Importance+of+gastrin+in+the+pathogenesis+and+treatment+of+gastric+tumors.">Importance of gastrin in the pathogenesis and treatment of gastric tumors.</a> World Journal of Gastroenterology. 15 (1), 1-16 (2009).</li><li>Hayakawa, Y., Chang, W., Jin, G., Wang, T. C. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Gastrin+and+upper+GI+cancers.">Gastrin and upper GI cancers.</a> Current Opinion in Pharmacology. 31, 31-37 (2016).</li><li>Zhang, C. Z., He, M. X., Jin, L. W., Liu, W. D. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Effect+of+aluminum+phosphate+gel+combined+with+atropine+on+patients+with+acute+gastritis+and+its+effect+on+serum+gastrin+and+malondialdehyde+levels.">Effect of aluminum phosphate gel combined with atropine on patients with acute gastritis and its effect on serum gastrin and malondialdehyde levels.</a> International Journal of Digestive Diseases. 40 (2), 137-140 (2020).</li><li>Wang, Y. K., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Levels+of+malondialdehyde+in+the+gastric+juice:+its+association+with+Helicobacter+pylori+infection+and+stomach+diseases.">Levels of malondialdehyde in the gastric juice: its association with Helicobacter pylori infection and stomach diseases.</a> Helicobacter. 23 (2), e12460 (2018).</li><li>Turkkan, E., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Does+Helicobacter+pylori-induced+inflammation+of+gastric+mucosa+determine+the+severity+of+symptoms+in+functional+dyspepsia.">Does Helicobacter pylori-induced inflammation of gastric mucosa determine the severity of symptoms in functional dyspepsia.</a> Journal of Gastroenterology. 44 (1), 66-70 (2009).</li><li>Liu, Y., Cai, C., Qin, X. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Regulation+of+gut+microbiota+of+Astragali+Radix+in+treating+for+chronic+atrophic+gastritis+rats+based+on+metabolomics+coupled+with+16S+rRNA+gene+sequencing.">Regulation of gut microbiota of Astragali Radix in treating for chronic atrophic gastritis rats based on metabolomics coupled with 16S rRNA gene sequencing.</a> Chemico-Biological Interactions. 365, 110063 (2022).</li><li>Gai, X., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Heptadecanoic+acid+and+pentadecanoic+acid+crosstalk+with+fecal-derived+gut+microbiota+are+potential+non-invasive+biomarkers+for+chronic+atrophic+gastritis.">Heptadecanoic acid and pentadecanoic acid crosstalk with fecal-derived gut microbiota are potential non-invasive biomarkers for chronic atrophic gastritis.</a> Frontiers in Cellular and Infection Microbiology. 12, 1064737 (2023).</li><li>Sgambato, D., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Gut+microbiota+and+gastric+disease.">Gut microbiota and gastric disease.</a> Minerva Gastroenterologica e Dietologica. 63 (4), 345-354 (2017).</li><li>Li, J., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Pharmacogenetic+study+of+Desmodium+caudatum.">Pharmacogenetic study of Desmodium caudatum.</a> Anais Da Academia Brasileira De Ciencias. 91 (2), e20180637 (2019).</li><li>Xu, Q. N., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Phenolic+glycosides+and+flavonoids+with+antioxidant+and+anticancer+activities+from+Desmodium+caudatum.">Phenolic glycosides and flavonoids with antioxidant and anticancer activities from Desmodium caudatum.</a> Natural Product Research. 35 (22), 4534-4541 (2021).</li><li>Li, W., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Anti-inflammatory+and+antioxidant+activities+of+phenolic+compounds+from+Desmodium+caudatum+leaves+and+stems.">Anti-inflammatory and antioxidant activities of phenolic compounds from Desmodium caudatum leaves and stems.</a> Archives of Pharmacal Research. 37 (6), 721-727 (2014).</li><li>Shao, X. H., Wang, J. G. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Establishment+of+chronic+atrophic+gastritis+in+a+rat+model.">Establishment of chronic atrophic gastritis in a rat model.</a> Journal of Zhangjiakou Medical Collage. 19 (2), 11-13 (2002).</li><li>Yu, C., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Dysbiosis+of+gut+microbiota+is+associated+with+gastric+carcinogenesis+in+rats.">Dysbiosis of gut microbiota is associated with gastric carcinogenesis in rats.</a> Biomedicine &amp; Pharmacotherapy. 126, 110036 (2020).</li><li>Chen, R., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Fecal+metabolomics+combined+with+16S+rRNA+gene+sequencing+to+analyze+the+changes+of+gut+microbiota+in+rats+with+kidney-yang+deficiency+syndrome+and+the+intervention+effect+of+You-gui+pill.">Fecal metabolomics combined with 16S rRNA gene sequencing to analyze the changes of gut microbiota in rats with kidney-yang deficiency syndrome and the intervention effect of You-gui pill.</a> Journal of Ethnopharmacology. 224, 112139 (2019).</li><li>Li, Q., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Magnetic+anchoring+and+guidance-assisted+endoscopic+irreversible+electroporation+for+gastric+mucosal+ablation:+a+preclinical+study+in+canine+model.">Magnetic anchoring and guidance-assisted endoscopic irreversible electroporation for gastric mucosal ablation: a preclinical study in canine model.</a> Surgical Endoscopy. 35 (10), 5665-5674 (2021).</li><li>Xu, H., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Therapeutic+assessment+of+fractions+of+Gastrodiae+Rhizoma+on+chronic+atrophic+gastritis+by+1H+NMR-based+metabolomics.">Therapeutic assessment of fractions of Gastrodiae Rhizoma on chronic atrophic gastritis by 1H NMR-based metabolomics.</a> Journal of Ethnopharmacology. 254, 112403 (2020).</li><li>Zhang, B. N., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Effects+of+Atractylodes+lancea+extracts+on+intestinal+flora+and+serum+metabolites+in+mice+with+intestinal+dysbacteriosis.">Effects of Atractylodes lancea extracts on intestinal flora and serum metabolites in mice with intestinal dysbacteriosis.</a> Proteome Science. 21 (1), 5 (2023).</li><li>Tian, H., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=The+therapeutic+effects+of+Magnolia+officinalis+extraction+on+an+antibiotics-induced+intestinal+dysbacteriosis+in+mice.">The therapeutic effects of Magnolia officinalis extraction on an antibiotics-induced intestinal dysbacteriosis in mice.</a> Current Microbiology. 77 (9), 2413-2421 (2020).</li><li>Wang, J., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Tumor+and+microecology+committee+of+China+anti-cancer+association.+Chinese+expert+consensus+on+intestinal+microecology+and+management+of+digestive+tract+complications+related+to+tumor+treatment+(version+2022).">Tumor and microecology committee of China anti-cancer association. Chinese expert consensus on intestinal microecology and management of digestive tract complications related to tumor treatment (version 2022).</a> Journal of Cancer Research and Therapeutics. 18 (7), 1835-1844 (2022).</li><li>Xu, W., Xu, L., Xu, C. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Relationship+between+Helicobacter+pylori+infection+and+gastrointestinal+microecology.">Relationship between Helicobacter pylori infection and gastrointestinal microecology.</a> Frontiers in Cellular and Infection Microbiology. 12, 938608 (2022).</li><li>Johnson, J. S., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Evaluation+of+16S+rRNA+gene+sequencing+for+species+and+strain-level+microbiome+analysis.">Evaluation of 16S rRNA gene sequencing for species and strain-level microbiome analysis.</a> Nature Communications. 10 (1), 5029 (2019).</li><li>Callahan, B. J., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=High-throughput+amplicon+sequencing+of+the+full-length+16S+rRNA+gene+with+single-nucleotide+resolution.">High-throughput amplicon sequencing of the full-length 16S rRNA gene with single-nucleotide resolution.</a> Nucleic Acids Research. 47 (18), e103 (2019).</li><li>Langille, M. G., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Predictive+functional+profiling+of+microbial+communities+using+16S+rRNA+marker+gene+sequences.">Predictive functional profiling of microbial communities using 16S rRNA marker gene sequences.</a> Nature Biotechnology. 31 (9), 814-821 (2013).</li><li>Huang, D., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Characteristics+of+intestinal+flora+in+patients+with+gastric+cancer+based+on+high+throughput+sequencing+technology.">Characteristics of intestinal flora in patients with gastric cancer based on high throughput sequencing technology.</a> Chinese Journal of Clinical Research. 35 (3), 303-306 (2022).</li><li>Shi, Y., Luo, J., Narbad, A., Chen, Q. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Advances+in+lactobacillus+restoration+for+&#946;-lactam+antibiotic-induced+dysbiosis:+a+system+review+in+intestinal+microbiota+and+immune+homeostasis.">Advances in lactobacillus restoration for &#946;-lactam antibiotic-induced dysbiosis: a system review in intestinal microbiota and immune homeostasis.</a> Microorganisms. 11 (1), 179 (2023).</li><li>Grigor'eva, I. N. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Gallstone+disease,+obesity+and+the+Firmicutes/Bacteroidetes+ratio+as+a+possible+biomarker+of+gut+dysbiosis.">Gallstone disease, obesity and the Firmicutes/Bacteroidetes ratio as a possible biomarker of gut dysbiosis.</a> Journal of Personalized Medicine. 11 (1), 13 (2020).</li><li>Thorne, G. M. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Diagnosis+of+infectious+diarrheal+diseases.">Diagnosis of infectious diarrheal diseases.</a> Infectious Disease Clinics of North America. 2 (3), 747-748 (1988).</li><li>Banks, M., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=British+society+of+gastroenterology+guidelines+on+the+diagnosis+and+management+of+patients+at+risk+of+gastric+adenocarcinoma.">British society of gastroenterology guidelines on the diagnosis and management of patients at risk of gastric adenocarcinoma.</a> Gut. 68 (9), 1545-1575 (2019).</li><li>Roy-Lachapelle, A., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Evaluation+of+ELISA-based+method+for+total+anabaenopeptins+determination+and+comparative+analysis+with+on-line+SPE-UHPLC-HRMS+in+freshwater+cyanobacterial+blooms.">Evaluation of ELISA-based method for total anabaenopeptins determination and comparative analysis with on-line SPE-UHPLC-HRMS in freshwater cyanobacterial blooms.</a> Talanta. 223, 121802 (2021).</li><li>Shahi, S. K., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Microbiota+analysis+using+two-step+PCR+and+next-generation+16S+rRNA+gene+sequencing.">Microbiota analysis using two-step PCR and next-generation 16S rRNA gene sequencing.</a> Journal of Visualized Experiments. (152), e59980 (2019).</li><li>Huang, R., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Blocking-free+ELISA+using+a+gold+nanoparticle+layer+coated+commercial+microwell+plate.">Blocking-free ELISA using a gold nanoparticle layer coated commercial microwell plate.</a> Sensors (Basel). 18 (10), 3537 (2018).</li></ol>

D. caudatum, a commonly used folk medicine by the Qiang nationality12, has shown significant efficacy in treating gastrointestinal diseases. With the advancement of modern pharmacological research, the imbalance of flora resulting from gastrointestinal microecology imbalance is identified as a key factor in acute and chronic gastrointestinal diseases22,23. Certain microorganisms in the intestinal tract play a crucial role in maint...

Chronic gastritis (CG), one of the most common clinical diseases, is characterized by chronic and persistent inflammatory changes in the gastric mucosa epithelium, which is frequently and repeatedly attacked by various pathogenic factors1. The incidence rate of CG ranks first among all types of stomach diseases, accounting for 40% to 60% of the outpatient service rate in the Department of Gastroenterology2. Moreover, the incidence rate generally increases with age, especially in individuals who are middle-aged and older3. Undoubtedly, CG significantly reduces people&#39;s quality of life, emphasizing the critical need to discover new therapeutic agents.
Numerous studies have reported that the occurrence and development of CG are linked to the secretion of gastrointestinal hormones, such as gastrin (GAS)4,5. GAS, a common gastrointestinal peptide hormone in the digestive tract, stimulates cells to secrete gastric acid by promoting the release of histamine from eosinophils. Additionally, it improves the nutrition and blood supply of the gastric mucosa, promoting the proliferation of gastric mucosa and parietal cells6. Therefore, gastrin can be utilized as an indicator to evaluate the development level of CG. Furthermore, lipid peroxidation products triggered by reactive oxides can activate inflammatory cells, leading to CG. Malondialdehyde (MDA), a lipid peroxidation marker, is a commonly used indicator to measure the degree of oxidative stress. It reflects the level of free radicals in the gastric mucosa to a certain extent. The MDA level can indicate the attack of unsaturated fatty acids in the local gastric mucosa caused by free radicals7,8.
Intestinal microecology consists of millions of microorganisms residing in the host gut, playing a vital role in maintaining host health and regulating host immunity. A healthy intestinal flora, characterized by high richness, diversity, and stable microbiota function, acts as a protective barrier against the invasion of pathogenic microorganisms by participating in metabolism. Disturbances in the intestinal flora make individuals more susceptible to acute and chronic gastrointestinal diseases9,10. In recent years, microbiota therapy for gastrointestinal diseases has progressed rapidly and demonstrated significant efficacy11. In summary, the consideration of intestinal flora is crucial in understanding and addressing gastrointestinal diseases.
As an indispensable component of the treasure trove of traditional Chinese medicine, folk medicinal materials hold great significance for clinical practice and the modernization of national medicine. The roots and the entire parts of Desmodium caudatum (Thunb.) DC. has been widely used for relieving stomach discomfort in the Beichuan Qiang area of Mianyang City for an extended period. Some articles indicate the scientific basis for treating gastrointestinal diseases with D. caudatum, citing its effects such as hemostasis, anti-oxidation, and gastrointestinal protection12,13,14. However, due to the lack of in-depth research, no clinical pharmacodynamics mechanism has been established. This article aims to study the effects of D. caudatum in treating gastritis based on gastrointestinal hormones and intestinal flora, providing a basis for its rational clinical application.

<table border="1" fo:keep-together.within-page="1" fo:keep-with-next.within-page="always">
	<tbody>
		<tr>
			<td>Alpha diversity analysis</td>
			<td>Mothur 1.30.2</td>
			<td></td>
			<td></td>
		</tr>
		<tr>
			<td>AxyPrep deoxyribonucleic acid (DNA) gel extraction kit&#160;</td>
			<td>Axygen Biosciences&#160;</td>
			<td>AP-GX-50</td>
			<td></td>
		</tr>
		<tr>
			<td>Beta diversity analysis</td>
			<td>Qiime 1.9.1</td>
			<td></td>
			<td></td>
		</tr>
		<tr>
			<td>Cryogenic refrigerator</td>
			<td>Forma-86C ULT freezer</td>
			<td></td>
			<td></td>
		</tr>
		<tr>
			<td>Desmodium caudatum</td>
			<td>The Traditional Chinese Medicine Hospital of Beichuan Qiang Autonomous County</td>
			<td></td>
			<td></td>
		</tr>
		<tr>
			<td>E.Z.N.A. soil kit</td>
			<td>Omega Bio-tek</td>
			<td>D5625-01</td>
			<td></td>
		</tr>
		<tr>
			<td>Illumina MiSeq Platform</td>
			<td>Illumina Miseq PE300/NovaSeq PE250</td>
			<td></td>
			<td></td>
		</tr>
		<tr>
			<td>Multiskan Spectrum</td>
			<td>spectraMax i3</td>
			<td></td>
			<td></td>
		</tr>
		<tr>
			<td>OTU clustering</td>
			<td>Uparse 7.0.1090</td>
			<td></td>
			<td></td>
		</tr>
		<tr>
			<td>OTU statistics</td>
			<td>Usearch 7.0</td>
			<td></td>
			<td></td>
		</tr>
		<tr>
			<td>PCR instrument</td>
			<td>TransGen AP221-02</td>
			<td></td>
			<td></td>
		</tr>
		<tr>
			<td>PCR instrument</td>
			<td>ABI GeneAmp 9700</td>
			<td></td>
			<td></td>
		</tr>
		<tr>
			<td>QuantiFluor-ST double-stranded DNA (dsDNA) system</td>
			<td>Promega Corp.</td>
			<td></td>
			<td></td>
		</tr>
		<tr>
			<td>Sequence classification annotation</td>
			<td>RDP Classifier 2.11</td>
			<td></td>
			<td></td>
		</tr>
		<tr>
			<td>Sodium salicylate</td>
			<td>Sichuan Xilong Chemical Co., Ltd</td>
			<td>54-21-7</td>
			<td></td>
		</tr>
		<tr>
			<td>SPF rats</td>
			<td>Chengdu Dashuo Experimental Animal Co., Ltd</td>
			<td></td>
			<td></td>
		</tr>
		<tr>
			<td>SPSS 18.0</td>
			<td>IBM</td>
			<td></td>
			<td></td>
		</tr>
	</tbody>
</table>

effects of desmodium caudatum on gastrointestinal hormones and intestinal flora in rats with gastritis

In order to preliminarily explore the effects of Desmodium caudatum on gastritis and intestinal flora in rats, a chronic gastritis rat model was established using the classic sodium salicylate method. Eighteen SPF rats were divided into three groups: the control group (Group C), the model group (Group M), and the treatment group (Group T). Pathological sections of the gastric wall were taken from rats in each group. Furthermore, the concentrations of gastrin and malondialdehyde in the serum of rats in each group were determined by ELISA. Additionally, the effects of D. caudatum on the intestinal flora of rats with gastritis were explored through a detailed comparison of gut bacterial communities in the three groups, employing Illumina-based 16S rRNA gene sequencing. The results indicated that D. caudatum decoction could reduce the malondialdehyde content and increase the gastrin content. Moreover, D. caudatum decoction was found to enhance the diversity and abundance of intestinal flora, exerting a positive impact on the treatment of gastritis by regulating and restoring the intestinal flora.

Author Spotlight: Exploring the Mysteries of Sichuan&#039;s Herbal Medicine in Chinese Medicine Research

Effects of Desmodium caudatum on Gastrointestinal Hormones and Intestinal Flora in Rats with Gastritis

As an indispensable component of the treasure trove of traditional Chinese medicine, folk medical materials hold great significance for clinical practice and modernization of national medicine. This article aims to study the effects of D.caudatum in treating gastritis based on gastrointestinal hormones and intestinal flora, providing a basis for its addition in clinical application. Numerous studies have reported that the occurrence and development of chronic gastric are linked to the secretion of gastrointestinal hormones, such as gastrin.It can be optimized as an indicator to evaluate the developmental level of chronic gastric. Furthermore, repeat peroxidation products triggered by reactive oxides can activate inflammatory cells, leading to chronic gastral. In 16S rRNA high throughput gene sequencing, low resolution may make it challenging to distinguish strains and genera with highly similar sequences, leading to difficulties in classification at higher taxonomic levels such as genus, family, and phylum.For the ELISA kit method, sample pretreatment steps like dilution and washing are usually required, introducing potential errors and variabilities.

The results of the pathological section of the stomach wall are depicted in Figure 1. In comparison to Group C, Group M exhibited mild gastric wall atrophy and mild inflammation. However, when compared to Group M, Group T showed no evident inflammation, intestinal metaplasia, or atrophy. This suggests that D. caudatum decoction can effectively improve gastritis.
The serum gastrointestinal hormone assay results are presented in Figure ...

Watch this Scientific Journal Video about Author Spotlight: Exploring the Mysteries of Sichuan's Herbal Medicine in Chinese Medicine Research at JoVE.com

The present protocol describes the method for analyzing intestinal flora using Illumina-based 16S rRNA gene sequencing and provides a framework for evaluating the effectiveness of herbal decoctions.

In order to preliminarily explore the effects of Desmodium caudatum on gastritis and intestinal flora in rats, a chronic gastritis rat model was ...

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Evaluating the Therapeutic Effects of Desmodium caudatum Decoction on Gastric Ailments in Rats

307 Views.  Mianyang Normal University.  The present protocol describes the method for analyzing intestinal flora using Illumina-based 16S rRNA gene sequencing and provides a framework for evaluating the effectiveness of herbal decoctions.

Desmodium caudatum Decoction's Impact on Intestinal Microbiota of Rats

概要