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Copenhagen University Hospital - Rigshospitalet

2 ARTICLES PUBLISHED IN JoVE

Medicine

Dual Test Gas Pulmonary Diffusing Capacity Measurement During Exercise in Humans Using the Single-Breath Method

Stine B. Nymand¹, Jacob Peter Hartmann^1,2,3, Helene Louise Hartmeyer¹, Iben E. Rasmussen¹, Amalie Bach Andersen¹, Milan Mohammad¹, Susan Al-Atabi³, Birgitte Hanel³, Ulrik Winning Iepsen^1,4, Jann Mortensen^3,5, Ronan M. G. Berg^1,2,3,6

¹Centre for Physical Activity Research, Copenhagen University Hospital - Rigshospitalet, ²Department of Biomedical Sciences, Faculty of Health and Medical Sciences, University of Copenhagen, ³Department of Clinical Physiology and Nuclear Medicine, Copenhagen University Hospital - Rigshospitalet, ⁴Department of Anesthesiology and Intensive Care, Hvidovre Hospital, ⁵Department of Clinical Medicine, Faculty of Health and Medical Sciences, University of Copenhagen, ⁶Neurovascular Research Laboratory, Faculty of Life Sciences and Education, University of South Wales

This protocol presents a method to assess pulmonary alveolar-capillary reserve measured by combined single-breath measurement of the diffusing capacity to carbon monoxide (D_L,CO) and nitric oxide (D_L,NO) during exercise. Assumptions and recommendations for using the technique during exercise form the foundation of this article.

Bioengineering

Surgical Model for Single-Staged Tissue-Engineered Urothelial Tubes in Minipigs

Nikolai Juul^1,2, Oliver Willacy^1,2, Anastasia Buch Kjeldgaard^1,2, Dennis Rootsi³, Karsten Hammelev⁴, Clara Ibel Chamorro³, Magdalena Fossum^1,2,3

¹Division of Pediatric Surgery, Department of Surgery and Transplantation, Copenhagen University Hospital - Rigshospitalet, ²Laboratory of Tissue Engineering, Department of Clinical Medicine, University of Copenhagen, ³Laboratory of Tissue Engineering, Department of Women's and Children's Health, Karolinska Institutet, ⁴Department of Experimental Medicine, University of Copenhagen

Tissue-engineered implants for reconstructive surgery rarely progress beyond preclinical trials due to laborious ex vivo culturing, which includes complex and expensive scaffold components. Here, we present a single-staged procedure designed for urinary diversion with an accessible collagen-based tubular scaffold containing autologous micrografts.