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University of Miami, Rosenstiel School of Marine and Atmospheric Sciences

2 ARTICLES PUBLISHED IN JoVE

Environment

In Situ Hybridization Techniques for Paraffin-Embedded Adult Coral Samples

¹Department of Marine Biology and Ecology, University of Miami, Rosenstiel School of Marine and Atmospheric Sciences

The goal of this protocol is to perform in situ hybridization on adult coral samples that have been embedded in paraffin and sectioned onto glass slides. This is a qualitative method used to visualize the spatial expression of an RNA anti-sense probe in paraffin-embedded tissues.

Biology

Fluorescence-Activated Cell Sorting for the Isolation of Scleractinian Cell Populations

Grace A. Snyder¹, William E. Browne², Nikki Traylor-Knowles^*1, Benyamin Rosental^*3

¹Department of Marine Biology and Ecology, Rosenstiel School of Marine and Atmospheric Science, University of Miami, ²Department of Biology, University of Miami, ³The Shraga Segal Department of Microbiology, Immunology, and Genetics, Faculty of Health Sciences, and Regenerative Medicine and Stem Cell Research Center, Ben Gurion University of the Negev

Corals create biodiverse ecosystems important for both humans and marine organisms. However, we still do not understand the full potential and function of many coral cells. Here, we present a protocol developed for the isolation, labeling, and separation of stony coral cell populations.