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Institut National de la Santé et de la Recherche Médicale (INSERM), UMR-S 1270 Paris, France

1 ARTICLES PUBLISHED IN JoVE

Neuroscience

Two-photon Imaging of Microglial Processes' Attraction Toward ATP or Serotonin in Acute Brain Slices

Fanny Etienne^1,2,3, Vincenzo Mastrolia^1,2,3, Luc Maroteaux^1,2,3, Jean-Antoine Girault^1,2,3, Nicolas Gervasi^*1,2,3, Anne Roumier^*1,2,3

¹Institut National de la Santé et de la Recherche Médicale (INSERM), UMR-S 1270 Paris, France, ²Sorbonne Université, Paris, France, ³Institut du Fer à Moulin, Paris, France

Microglia, the resident immune cells of the brain, respond quickly with morphological changes to modifications of their environment. This protocol describes how to use two-photon microscopy to study the attraction of microglial processes toward serotonin or ATP in acute brain slices of mice.