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Constructing the 3D Human-Stem-Cell-Derived In Vitro Blood Brain Barrier

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02:08 min

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October 20th, 2023

DOI :

10.3791/200668-v

October 20th, 2023

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105 Views

Camille Goldman*¹^,²^,³^,⁴^,⁵, Natalie Suhy*¹^,²^,³^,⁴^,⁵, Jessica E. Schwarz¹^,²^,³^,⁴^,⁵, Emily Ruth Sartori¹^,²^,³^,⁴^,⁵, Rikki B. Rooklin¹^,²^,³^,⁴^,⁵, Braxton R. Schuldt¹^,²^,³^,⁴^,⁵, Louise A. Mesentier-Louro¹^,²^,³^,⁴^,⁵, Joel W. Blanchard¹^,²^,³^,⁴^,⁵

¹Icahn School of Medicine at Mount Sinai, ²Black Family Stem Cell Institute at Mount Sinai, ³Ronald M. Loeb Center for Alzheimer’s Disease at Mount Sinai, ⁴Friedman Brain Institute at Mount Sinai, ⁵Nash Family Department of Neuroscience at Mount Sinai

* These authors contributed equally

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Keywords 3D Human stem cell derived

In Vitro Blood brain Barrier

Dissociated Endothelial Cells

Reduced GF Basement Membrane Matrix

Cell Matrix Mixture

48 well Or 96 well Glass bottom Culture Dish

Polymerize The Matrix

Supplemented Astrocyte Medium

Downstream Assays

Properly Formed IBBB

Single Translucent Disk

シリーズから

A 3D Blood-Brain Barrier Model Derived from Human Pluripotent Stem-Cells

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