Limit of Detection (LOD) Assay to Check the Sensitivity of DNAzyme-Based Nanomachines (DNM)

To begin, design DNAzyme-based nano machines, or DNM, with a 10 to 23 core, using free software. Assemble the DNM and check its size and homogeneity by native page. Then prepare 160 microliters of F-sub in the reaction buffer.

Prepare seven