Setting Up and Estimating the Pellicle Biofilm Development in Mycobacterium smegmatis Cultures

To begin, in a laminar hood, dispense six milliliters of Soutan's media supplemented with 2%glucose into the wells of a six-well plate. Inoculate the wells with 120 microliters of the secondary cultures of mycobacterium smegmatis. Keep one un-inoc