Staining the Antigen-Specific T Cells with Peptide:MHC Tetramers for Flow Cytometry Analysis and Sorting

After enriching the lung sample for T cells, add one milliliter of 10 millimolar dasatinib to each sample. Vortex the mixture and incubate for five minutes at room temperature. Add PE or APC conjugated peptide MHC tetramer to a final concentration