Preparing Cells Expressing Rapamycin-Regulated Phosphatase for Immunoprecipitation to Assess Temporal Control of Tyrosine Phosphatases

To begin, obtain HEK 293T cells and plate 800, 000 cells per well in a 6-well tissue culture plate in supplemented DMEM media. Incubate the plate overnight at 37 degrees Celsius with 5%carbon dioxide to achieve 60 to 80%confluency. Next, mix one m