Phosphatase Activity Assay to Characterize Allosteric Regulation of Phosphatases with the Rapamycin-Regulated RapR System

To begin, obtain the HEK293T cells expressing RapR-Shp2 and perform immunoprecipitation with Protein G-Sepharose. After immunoprecipitation, wash the sepharose two times each with 0.5 milliliters of lysis buffer, followed by 0.5 milliliters of was