Heat Stress Treatment of Arabidopsis Seedlings and Sucrose Gradient Preparation for Polysome Profiling and RNA Extraction

To begin, using a dropper, plate 250 Arabidopsis seeds for each group of conditions on filter paper placed on a Murashige and Skoog Basal Media agar plate without sucrose, supplemented with 1.2%phyto agar. Wrap the plate with aluminum foil to bloc