JoVE Journal

Immunology and Infection

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Antigen Specific In Vivo Killing Assay using CFSE Labeled Target Cells

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09:49 min

November 9th, 2010

November 9th, 2010

19,398 Views

0:00

Title

1:13

Habituating Animals

1:29

Eliciting Target-Specific Effector CTLs by Immunization (Day 0)

3:03

Program Operant Test Session

3:16

Harvesting Target Cells (Day 8)

5:32

Treatment and Labeling of Target Cells

6:33

Results: Operant Sensation Seeking Behavior

7:39

Adoptive Transfer and Subsequent Harvest of Labeled Cells

8:06

Conclusion

8:20

Flow Cytometry and Data Analysis

8:36

CFSE Profile Demonstrating Cell-Specific Killing in BALB/c Mice

9:34

Conclusion

Many infections elicit a strong CTL response, but occasionally, the quantity of responding cells does not correlate to control of the pathogen1. One measure of CTL quality is their ability to kill specifically2. CFSE labeling of target cells can be used to investigate this CTL response quality in vivo3,4.

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