我々は、この原稿のために書き上げるの彼女の貴重な入力のために博士メアリー·ルー·Vallano、神経科学と生理学教室の取り組みを承認したいと思います。

1。 0.15ミリリットル自家動脈血を注射SAH Subjectのラットの手術<ol><li>ラットでは、ケタミン/キシラジンげっ歯類のカクテルの0.1 mg / kgを使用し、5分間静置し、麻酔をかける。 </li><li>十分な麻酔が後肢反射の減少によって確認された。 </li><li>サブ後頭部周りの毛の鼻の領域に首を剃られている電子シェーバーを使用しています。 </li><li>動物は、手術台上に仰臥位に置かれ、尾は無菌切開を確保するためにベタジンとスワブされています。 </li><li>ストレート1cmの正中切開を尾の腹側面に取られる</li><li>尾動脈が同定され単離されるまで切開が延長されます。 </li><li>滅菌26ゲージのカテーテルを使用して、尾動脈にカニューレを挿入され、動脈血の0.15ミリリットルを注射器に引き込まれる。 </li><li>滅菌ガーゼは、切開部を封止するvetbondの適用前に、止血を確実にするために切開の周りにラップされます。 </li><li> T彼ラットはベタジンとスワブれるサブ後頭部オーバーテーブルと剃らエリアで発生しやすいになっています。 </li><li>垂直正中切開·アクセスを使用すると大槽に得られる。 </li><li>一旦同定さ25ゲージの針を大槽内に挿入され、CSFの0.15ミリリットル、自家血液量の注入による増加頭蓋内圧力を回避するために、注射器に引き込まれる。 </li><li>さて、尾動脈から抽出された血液の0.15ミリリットルを大槽にゆっくりと注入される。 </li><li> 30秒は、くも膜下腔に血液凝固を確認してから、慎重に撤回するために針を付けたままにしています。 </li><li>止血が確保され、切開はステープルデバイスを使用して閉じられます。 </li><li>動物は、今や脳底動脈の周りに水槽に凍らせるために血液を許可するように20分間下の位置20°ヘッドと温暖化の面に配置されている傾向がある。 </li><li> 1.1から1.15までの手順がバラバラに二度目の手術の48時間の間に繰り返されます。 </li&gt; </ol> 2。 0.15ミリリットルの生理食塩水を注入しSAH題目用ラットの手術<ol><li>ラットでは、ケタミン/キシラジンげっ歯類のカクテルの0.1 mg / kgを使用し、5分間静置し、麻酔をかける。 </li><li>十分な麻酔が後肢反射の減少によって確認された。 </li><li>サブ後頭部周りの毛の鼻の領域に首を剃られている電子シェーバーを使用しています。 </li><li>動物は、手術台上に仰臥位に置かれ、尾は無菌切開を確保するためにベタジンとスワブされています。 </li><li>ストレート1cmの正中切開を尾の腹側面に取られる</li><li>尾動脈が同定され単離されるまで切開が延長されます。 </li><li>滅菌26ゲージのカテーテルを使用して、尾動脈にカニューレを挿入され、動脈血の0.15ミリリットルを注射器に引き込まれる。 </li><li>滅菌ガーゼは、切開部を封止するvetbondの適用前に、止血を確実にするために切開の周りにラップされます。 </li><li> RAtは、ベタジンで描かれているサブ後頭部の上にテーブルと剃ら領域に発生しやすいになっています。 </li><li>垂直正中切開·アクセスを使用すると大槽に得られる。 </li><li>一旦同定さ25ゲージの針を大槽内に挿入され、CSFの0.15ミリリットルを注射器内に引き込まれ、サンプルが格納されます。 </li><li>さて、通常の生理食塩水の0.15ミリリットル（37°C）を大槽にゆっくりと注入される。 </li><li>針は30秒間そのままにして慎重に引き抜かれる。 </li><li>止血が確保され、切開はステープルデバイスを使用して閉じられます。 </li><li>動物は、今や20分間ポジションダウン20°ヘッドと温暖化の面に配置されている傾向がある。 </li><li> 2.1から2.15までの手順がバラバラに二度目の手術の48時間の間に繰り返されます。 </li></ol> 3。ラットサクリファイス<ol><li>二度目の手術後5日目に、ラットは心臓灌流によって犠牲にされています。 </li><li>ラットは致死量（0が与えられている致命的なプラス（VORTECHファーマ株式会社、ディアボーン、ミシガン州）の0.​​2ミリリットル/キログラム） </li><li>垂直正中切開で、腹腔に接近され、腹膜が開かれます。 </li><li>前方開胸が行われ、心臓を露出させる。 </li><li>リン酸緩衝液（PBS pH7.4及び37℃）に接続され、26ゲージのカテーテルを用いた動物は、血液の排出された後、4％パラホルムアルデヒドで灌流される。 </li><li>十分な血流を確保した後、血流が停止され、ラットを断頭台に引き渡されます。 </li><li>断頭後、骨骨鉗子は、脳の除去のための頭蓋を除去するために使用されます。 </li><li>脳と脳幹は慎重に48時間頭蓋から抽出され、4％パラホルムアルデヒド溶液に入れ、4℃で保存されています。 </li></ol> 4。攣縮を評価するためのセクションの作成<ol><li>今では4日間の30％スクロースに沈めれたラットの脳は目に持って来られるセクショニングのeはクライオスタット。 </li><li>一度凍結保護し、12μmの切片を、被験者間の一貫性を確保するための出発点として前下小脳動脈（AICA）で、クライオスタットを使用して作成します。 </li><li> 20セクションは上小脳動脈（SCA）で終わる、それぞれの動物のために作成されます。 </li><li>切片はスライドガラス上に置き、組織学的手法を用いて血管攣縮のために評価が行われ</li></ol>

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我々は、この研究に係る開示することは何もありません。

げっ歯8よりも類似遺伝子組成と人間への解剖学的特徴を有する霊長類、より密接に遅延した脳血管攣縮のイベントを模倣し、より簡単に動脈の変更を監視する非侵襲的な画像診断装置（MRI、血管造影検査）を受けることができ、。しかし、霊長類モデルではコスト的に不可能と小動物モデルよりも複雑なケアと倫理的な問題に関連付けられています。開発されてきた小動物SAHモデル?...

<table border="1"><tbody><tr><td> 機器/試薬の名称 </td><td> 会社 </td><td> カタログ番号 </td></tr><tr><td> SD系雄性ラット（250〜300 g）を</td><td>タコニック</td><td> SD-M </td></tr><tr><td> 26 Gのカテーテル</td><td>ウェブスター</td><td> 8416683 </td></tr><tr><td> 25 G針</td><td>バッファロー</td><td> 305122 </td></tr><tr><td> 1ccのシリンジ</td><td>中央ストア</td><td> 54245 </td></tr><tr><td>ケタミン/キシラジンカクテル</td><td>アニマルケア（SUNY）* </td><td> - </td></tr><tr><td>ベタジン</td><td>中央ストア</td><td> 51458 </td></tr><tr><td>スクロース</td><td>シグマ</td><td> S9378-1キロ</td></tr><tr><td>パラホルムアルデヒド</td><td>シグマ</td><td> P6148-500G </td></tr><tr><td>リン酸緩衝液</td><td>フィッシャー</td><td> BP-399から4 </td></tr><tr><td>手術台</td><td>ハーバード</td><td> PY2 72から2590 </td></tr><tr><td> OCTコンパウンド（cryoprotection） </td><td> VWR </td><td> 25608-930 </td></tr><tr><td> Superfrostスライド</td><td>フィッシャー</td><td> 12-550-15 </td></tr></tbody></table> *実験動物管理学科、ニューヨーク州立大学アップステート医科大学で合成。 1 ccを追加する[100 mg / mlの] [100 mg / mlの]ケタミンの10ミリリットルにキシラジンの。

a low mortality rat model to assess delayed cerebral vasospasm after experimental subarachnoid hemorrhage

目的：特性化開始イベント、病態生理学的変化や治療のための潜在的なターゲットを特定するために、ラットにおける動脈瘤性くも膜下出血（SAH）後の遅れの脳血管攣縮を示し、再現モデルを確立する。 方法：二十八雄Sprague-Dawleyラット（250 - 300gを） - くも膜下出血または生理食塩水対照任意に2つのグループのいずれかに割り当てられていた。 SAH群（n = 15）のラットくも膜下出血は、大槽内に、48時間離れて、自己血の二重注入によって誘発された。同様に、生理食塩水（N = 13）が大槽食塩水対照群の中に注入した。ラットは、第2の血液注射後5日目に屠殺し、脳を組織学的分析のために保存されていた。攣縮の程度は、NIHイメージ-Jのソフトウェアを使用して、内部の管腔の断面積を測定することにより、脳底動脈のセクションを使用して測定した。意義があったテューキー/クレイマーの統計分析を使用してテストされています。 結果：組織切片の分析後、脳底動脈内腔の断面積は、前者のグループの脳血管攣縮と一貫し、生理食塩水群よりもクモ膜下出血で小さかった。 SAH群、脳底動脈の内部領域（0.056μmの±3）（初期血注射後7日間）5日間第2の血液注射後血管攣縮から有意に小さかったで、内部領域と生理食塩水対照群と比較して（0.069 ±3、P = 0.004）。脳血管攣縮からの死亡は認められなかった。 結論：ラットダブルSAHモデルは、小さな動物モデルにおいて、脳血管攣縮の病態生理学的メカニズムを研究するために使用することができる穏やかな、サバイバル、脳底動脈の攣縮を誘発する。低く、許容死亡率は血管攣縮のメカニズムはelucidすることができるように理想的なSAHモデル動物に対して満足すべき重要な基準ですated 7,8。モデルの更なる修正が血管攣縮および神経学的試験の増加重症度を調整するために行うことができます。

上記のプロトコルの中で、我々が以前に文献に記載されているものよりも良いモデルの特性を必要とすると考えているいくつかの手順があります。ここでは、再現性の低死亡率脳血管攣縮小動物モデルを実現し、正しく行われない場合は、このモデルに関連付けられている潜在的な落とし穴を避けるために不可欠なステップに焦点を当てる。 1。尾動脈からの自己採血： </...

Watch this Scientific Journal Video about A Low Mortality Rat Model to Assess Delayed Cerebral Vasospasm After Experimental Subarachnoid Hemorrhage at JoVE.com

A Low Mortality Rat Model to Assess Delayed Cerebral Vasospasm After Experimental Subarachnoid Hemorrhage

動脈瘤性くも膜下出血（SAH）は時動脈瘤破裂くも膜下腔に発生する出血です。このイベントの罹患率と死亡率が改善された治療アプローチのために減少傾向にあったが、くも膜下出血後の脳血管攣縮のリスクは、それが数年前と同じであり続けている。脳血管攣縮の開始事象を識別するために、包括的かつ再現可能な動物モデルを確立することの重要性は、バリーが1979年に実験的脳血管攣縮モデルにおけるラットの最初の使用以来、研究の焦点となっている<em&gt;ら。</em&gt;ラットにおける初期の研究では、大槽内に自己血の単回注射は急性につながった（数分以内）ですが、脳血管攣縮を延期しないことを実証し<sup&gt; 3、6、14</sup&gt;。ここでは、再現性の遅発性脳血管攣縮の結果が低い死亡率くも膜下出血モデルラットを特徴付ける。

実験的くも膜下出血後の脳血管攣縮遅延を評価するための低死亡率のラットモデル

Objective: To characterize and establish a reproducible model that demonstrates delayed cerebral vasospasm after aneurysmal subarachnoid hemorrhage (SAH) ...

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14.2K ビュー.  SUNY Upstate Medical University. 動脈瘤性くも膜下出血（SAH）は時動脈瘤破裂くも膜下腔に発生する出血です。このイベントの罹患率と死亡率が改善された治療アプローチのために減少傾向にあったが、くも膜下出血後の脳血管攣縮のリスクは、それが数年前と同じであり続けている。脳血管攣縮の開始事象を識別するために、包括的かつ再現可能な動物モデルを確立することの重要性?...

ビデオ: A Low Mortality Rat Model to Assess Delayed Cerebral Vasospasm After Experimental Subarachnoid Hemorrhage

Modeling Stroke in Mice - Middle Cerebral Artery Occlusion with the Filament Model

Stroke is among the most frequent causes of death and adult disability, especially in highly developed countries. However, treatment options to date are very limited. To meet the need for novel therapeutic approaches, experimental stroke research frequently employs rodent models of focal cerebral ischaemia. Most researchers use permanent or transient occlusion of the middle cerebral artery (MCA) in mice or rats.
Proximal occlusion of the middle cerebral artery (MCA) via the intraluminal suture technique (so called filament or suture model) is probably the most frequently used model in experimental stroke research. The intraluminal MCAO model offers the advantage of inducing reproducible transient or permanent ischaemia of the MCA territory in a relatively non-invasive manner. Intraluminal approaches interrupt the blood flow of the entire territory of this artery. Filament occlusion thus arrests flow proximal to the lenticulo-striate arteries, which supply the basal ganglia. Filament occlusion of the MCA results in reproducible lesions in the cortex and striatum and can be either permanent or transient. In contrast, models inducing distal (to the branching of the lenticulo-striate arteries) MCA occlusion typically spare the striatum and primarily involve the neocortex. In addition these models do require craniectomy. In the model demonstrated in this article, a silicon coated filament is introduced into the common carotid artery and advanced along the internal carotid artery into the Circle of Willis, where it blocks the origin of the middle cerebral artery. In patients, occlusions of the middle cerebral artery are among the most common causes of ischaemic stroke. Since varying ischemic intervals can be chosen freely in this model depending on the time point of reperfusion, ischaemic lesions with varying degrees of severity can be produced. Reperfusion by removal of the occluding filament at least partially models the restoration of blood flow after spontaneous or therapeutic (tPA) lysis of a thromboembolic clot in humans.
In this video we will present the basic technique as well as the major pitfalls and confounders which may limit the predictive value of this model.

Filamentous occlusion of the Middle cerebral artery is a common model for studying ischemic stroke in mice.

マウスにおけるモデリングのストローク - フィラメントモデルと中大脳動脈閉塞

Stroke is among the most frequent causes of death and adult disability, especially in highly developed countries. However, treatment options to date are ...

医学

Non-invasive Imaging of Acute Allograft Rejection after Rat Renal Transplantation Using 18F-FDG PET

The number of patients with end-stage renal disease, and the number of kidney allograft recipients continuously increases. Episodes of acute cellular allograft rejection (AR) are a negative prognostic factor for long-term allograft survival, and its timely diagnosis is crucial for allograft function 1. At present, AR can only be definitely diagnosed by core-needle biopsy, which, as an invasive method, bares significant risk of graft injury or even loss. Moreover, biopsies are not feasible in patients taking anticoagulant drugs and the limited sampling site of this technique may result in false negative results if the AR is focal or patchy. As a consequence, this gave rise to an ongoing search for new AR detection methods, which often has to be done in animals including the use of various transplantation models.
Since the early 60s rat renal transplantation is a well-established experimental method for the examination and analysis of AR 2. We herein present in addition small animal positron emission tomography (PET) using 18F-fluorodeoxyglucose (FDG) to assess AR in an allogeneic uninephrectomized rat renal transplantation model and propose graft FDG-PET imaging as a new option for a non-invasive, specific and early diagnosis of AR also for the human situation 3. Further, this method can be applied for follow-up to improve monitoring of transplant rejection 4.

Non-invasive Imaging of Acute Allograft Rejection after Rat Renal Transplantation Using 18F-FDG PET

We herein present a rat renal transplantation model to non-invasively assess acute allograft rejection using positron emission tomography with 18F-fluorodeoxyglucose.

使用したラット腎移植後の急性拒絶反応の非侵襲的イメージング<sup&gt; 18</sup&gt; F-FDG PET

The number of patients with end-stage renal  disease, and the number of kidney allograft recipients continuously increases.  Episodes of acute cellular ...

Surgical Procedures for a Rat Model of Partial Orthotopic Liver Transplantation with Hepatic Arterial Reconstruction

Orthotopic liver transplantation (OLT) in rats using a whole or partial graft is an indispensable experimental model for transplantation research, such as studies on graft preservation and ischemia-reperfusion injury 1,2, immunological responses 3,4, hemodynamics 5,6, and small-for-size syndrome 7. The rat OLT is among the most difficult animal models in experimental surgery and demands advanced microsurgical skills that take a long time to learn. Consequently, the use of this model has been limited. Since the reliability and reproducibility of results are key components of the experiments in which such complex animal models are used, it is essential for surgeons who are involved in rat OLT to be trained in well-standardized and sophisticated procedures for this model.
While various techniques and modifications of OLT in rats have been reported 8 since the first model was described by Lee et al. 9 in 1973, the elimination of the hepatic arterial reconstruction 10 and the introduction of the cuff anastomosis technique by Kamada et al. 11 were a major advancement in this model, because they simplified the reconstruction procedures to a great degree. In the model by Kamada et al., the hepatic rearterialization was also eliminated. Since rats could survive without hepatic arterial flow after liver transplantation, there was considerable controversy over the value of hepatic arterialization. However, the physiological superiority of the arterialized model has been increasingly acknowledged, especially in terms of preserving the bile duct system 8,12 and the liver integrity 8,13,14.
In this article, we present detailed surgical procedures for a rat model of OLT with hepatic arterial reconstruction using a 50% partial graft after ex vivo liver resection. The reconstruction procedures for each vessel and the bile duct are performed by the following methods: a 7-0 polypropylene continuous suture for the supra- and infrahepatic vena cava; a cuff technique for the portal vein; and a stent technique for the hepatic artery and the bile duct.

Orthotopic liver transplantation in rats is an indispensable experimental model for biomedical research. Here we present our surgical procedures for orthotopic rat liver transplantation with hepatic arterial reconstruction using a 50% partial graft.

肝動脈再建を用いた部分同所性肝移植のラットモデルのための外科的処置

Orthotopic liver transplantation (OLT) in rats using a whole or partial graft is an indispensable experimental model for transplantation research, such as ...

Optimized System for Cerebral Perfusion Monitoring in the Rat Stroke Model of Intraluminal Middle Cerebral Artery Occlusion

The translational potential of pre-clinical stroke research depends on the accuracy of experimental modeling. Cerebral perfusion monitoring in animal models of acute ischemic stroke allows to confirm successful arterial occlusion and exclude subarachnoid hemorrhage. Cerebral perfusion monitoring can also be used to study intracranial collateral circulation, which is emerging as a powerful determinant of stroke outcome and a possible therapeutic target. Despite a recognized role of Laser Doppler perfusion monitoring as part of the current guidelines for experimental cerebral ischemia, a number of technical difficulties exist that limit its widespread use. One of the major issues is obtaining a secure and prolonged attachment of a deep-penetration Laser Doppler probe to the animal skull. In this video, we show our optimized system for cerebral perfusion monitoring during transient middle cerebral artery occlusion by intraluminal filament in the rat. We developed in-house a simple method to obtain a custom made holder for twin-fibre (deep-penetration) Laser Doppler probes, which allow multi-site monitoring if needed. A continuous and prolonged monitoring of cerebral perfusion could easily be obtained over the intact skull.

Cerebral perfusion monitoring has been demonstrated to improve accuracy in ischemic stroke models. Technical difficulties often limit the use of this essential tool for cerebrovascular research. In this video, an optimized system is shown to obtain a single or multi-site hemodynamic monitoring during intraluminal middle cerebral artery occlusion in rats.

腔内中大脳動脈閉塞のラット脳卒中モデルにおける脳血流モニタリングのための最適化されたシステム

The translational potential of pre-clinical stroke research depends on the accuracy of experimental modeling. Cerebral perfusion monitoring in animal ...

A Murine Model of Subarachnoid Hemorrhage

In this video publication a standardized mouse model of subarachnoid hemorrhage (SAH) is presented. Bleeding is induced by endovascular Circle of Willis perforation (CWp) and proven by intracranial pressure (ICP) monitoring. Thereby a homogenous blood distribution in subarachnoid spaces surrounding the arterial circulation and cerebellar fissures is achieved. Animal physiology is maintained by intubation, mechanical ventilation, and continuous on-line monitoring of various physiological and cardiovascular parameters: body temperature, systemic blood pressure, heart rate, and hemoglobin saturation. Thereby the cerebral perfusion pressure can be tightly monitored resulting in a less variable volume of extravasated blood. This allows a better standardization of endovascular filament perforation in mice and makes the whole model highly reproducible. Thus it is readily available for pharmacological and pathophysiological studies in wild type and genetically altered mice.

A standardized mouse model of subarachnoid hemorrhage by intraluminal Circle of Willis perforation is described. Vessel perforation and subarachnoid bleeding are monitored by intracranial pressure monitoring. In addition various vital parameters are recorded and controlled to maintain physiologic conditions.

くも膜下出血のマウスモデル

In this video publication a standardized mouse model of subarachnoid  hemorrhage (SAH) is presented. Bleeding is induced by endovascular Circle of  Willis ...

The Rabbit Blood-shunt Model for the Study of Acute and Late Sequelae of Subarachnoid Hemorrhage: Technical Aspects

Early brain injury and delayed cerebral vasospasm both contribute to unfavorable outcomes after subarachnoid hemorrhage (SAH). Reproducible and controllable animal models that simulate both conditions are presently uncommon. Therefore, new models are needed in order to mimic human pathophysiological conditions resulting from SAH.
This report describes the technical nuances of a rabbit blood-shunt SAH model that enables control of intracerebral pressure (ICP). An extracorporeal shunt is placed between the arterial system and the subarachnoid space, which enables examiner-independent SAH in a closed cranium. Step-by-step procedural instructions and necessary equipment are described, as well as technical considerations to produce the model with minimal mortality and morbidity. Important details required for successful surgical creation of this robust, simple and consistent ICP-controlled SAH rabbit model are described.

The experimental intracranial pressure-controlled blood shunt subarachnoid hemorrhage (SAH) model in the rabbit combines the standard procedures &#8212; subclavian artery cannulation and transcutaneous cisterna magna puncture, which enables close mimicking of human pathophysiological conditions after SAH. We present step-by-step instructions and discuss key surgical points for successful experimental SAH creation.

くも膜下出血の急性および後期後遺症の研究のためのウサギの血液シャントモデル：技術的側面

Early brain injury and delayed cerebral vasospasm both contribute to unfavorable outcomes after subarachnoid hemorrhage (SAH). Reproducible and ...

Leprdb Mouse Model of Type 2 Diabetes: Pancreatic Islet Isolation and Live-cell 2-Photon Imaging Of Intact Islets

Type 2 diabetes is a chronic disease affecting 382 million people in 2013, and is expected to rise to 592 million by 2035 1. During the past 2 decades, the role of beta-cell dysfunction in type 2 diabetes has been clearly established 2. Research progress has required methods for the isolation of pancreatic islets. The protocol of the islet isolation presented here shares many common steps with protocols from other groups, with some modifications to improve the yield and quality of isolated islets from both the wild type and diabetic Leprdb (db/db) mice. A live-cell 2-photon imaging method is then presented that can be used to investigate the control of insulin secretion within islets.

Leprdb Mouse Model of Type 2 Diabetes: Pancreatic Islet Isolation and Live-cell 2-Photon Imaging Of Intact Islets

We present here a protocol for the isolation of islets from the mouse model of type 2 diabetes, Leprdb and details of a live-cell assay for measurement of insulin secretion from intact islets that utilizes 2 photon microscopy.

LEPR<sup&gt; DB</sup&gt; 2型糖尿病のマウスモデル：無傷の膵島の膵島単離およびライブセル2光子イメージング

Type 2 diabetes is a chronic disease affecting 382 million people in 2013, and is expected to rise to 592 million by 2035 1. During the past 2 decades, ...

Integrated Compensatory Responses in a Human Model of Hemorrhage

Hemorrhage is the leading cause of trauma-related deaths, partly because early diagnosis of the severity of blood loss is difficult. Assessment of hemorrhaging patients is difficult because current clinical tools provide measures of vital signs that remain stable during the early stages of bleeding due to compensatory mechanisms. Consequently, there is a need to understand and measure the total integration of mechanisms that compensate for reduced circulating blood volume and how they change during ongoing progressive hemorrhage. The body&#39;s reserve to compensate for reduced circulating blood volume is called the &#39;compensatory reserve&#39;. The compensatory reserve can be accurately evaluated with real-time measurements of changes in the features of the arterial waveform measured with the use of a high-powered computer. Lower Body Negative Pressure (LBNP) has been shown to simulate many of the physiological responses in humans associated with hemorrhage, and is used to study the compensatory response to hemorrhage. The purpose of this study is to demonstrate how compensatory reserve is assessed during progressive reductions in central blood volume with LBNP as a simulation of hemorrhage.

The purpose of this protocol is to demonstrate the techniques for measuring compensatory responses to reduced central blood volume using lower body negative pressure as a noninvasive experimental model of human hemorrhage which can be used to quantify the total integration of compensatory mechanisms to blood volume deficit in humans.

出血の人体モデルで統合代償応答

Hemorrhage is the leading cause of trauma-related deaths, partly because early diagnosis of the severity of blood loss is difficult. Assessment of ...

Transplantation of Adipose Tissue-Derived Stem Cell Sheet to Reduce Leakage After Partial Colectomy in A Rat Model

Anastomotic leakage is a disastrous complication after colorectal surgery. Although current methods for leakage prevention have different levels of clinical efficacy, they are until now imperfect solutions. Stem cell therapy using ASC sheets could provide a solution to this problem. ASCs are considered as promising candidates for promoting tissue healing because of their trophic and immunomodulatory properties. Here, we provide methods to produce high-density ASC sheets, that are transplanted onto a colorectal anastomosis in a rat model to reduce the leakage. ASCs formed cell sheets in thermo-responsive culture dishes that could be easily detached. On the day of the transplantation, a partial colectomy with a 5-suture colorectal anastomosis was performed. Animals were immediately transplanted with 1 ASC sheet per rat. ASC sheets adhered spontaneously to the anastomosis without any glue, suture, or any biomaterial. Animal groups were sacrificed 3 and 7 days postoperatively. Compared to transplanted animals, the incidence of anastomotic abscesses and leakage was higher in control animals. In our model, the transplantation of ASC sheets after colorectal anastomosis was successful and associated with a lower leakage rate.

The preparation and transplantation of adipose tissue derived stem cell (ASC) sheets onto insufficiently sutured colorectal anastomoses in a rat model is presented. This novel application shows that ASC sheets can reduce colorectal anastomosis leakage.

ラットモデルにおける部分的な術後後の漏れを減らすために脂肪組織由来幹細胞シートの移植

Anastomotic leakage is a disastrous complication after colorectal surgery. Although current methods for leakage prevention have different levels of ...

Implantation of hiPSC-derived Cardiac-muscle Patches after Myocardial Injury in a Guinea Pig Model

Due to the limited regeneration capacity of the heart in adult mammals, myocardial infarction results in an irreversible loss of cardiomyocytes. This loss of relevant amounts of heart muscle mass can lead to the heart failure. Besides heart transplantation, there is no curative treatment option for the end-stage heart failure. In times of organ donor shortage, organ independent treatment modalities are needed. Left-ventricular assist devices are a promising therapy option, however, especially as destination therapy, limited by its side-effects like stroke, infections and bleedings. In recent years, several cardiac repair strategies including stem cell injection, cardiac progenitors or myocardial tissue engineering have been investigated. Recent improvements in cell biology allow for the differentiation of large amounts of cardiomyocytes derived from human induced pluripotent stem cells (iPSC). One of the cardiac repair strategies currently under evaluation is to transplant artificial heart tissue. Engineered heart tissue (EHT) is a three-dimensional in vitro created cardiomyocyte network, with functional properties of native heart tissue. We have created EHT-patches from hiPSC derived cardiomyocytes. Here we present a protocol for the induction of left ventricular myocardial cryoinjury in a guinea pig, followed by implantation of hiPSC derived EHT on the left ventricular wall.

Here we present a protocol for the induction of left ventricular cryoinjury followed by the implantation of a cardiac muscle patch, derived from human iPS-cell cardiomyocytes in a guinea pig model.

モルモット モデルにおける心筋傷害の後 - 心筋パッチのこと由来の注入

Due to the limited regeneration capacity of the heart in adult mammals, myocardial infarction results in an irreversible loss of cardiomyocytes. This loss ...