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In Vivo Nanovector Delivery of a Heart-specific MicroRNA-sponge

7.2K Views

09:53 min

June 15th, 2018

June 15th, 2018

7,250 Views

0:04

Title

0:56

Cloning the Recipient Vector to Include a Tissue-specific Promoter

3:48

Cloning the Sponge

4:55

Generation of Stable H9c2 miR-181-sponge Expressing Cells

5:53

Synthesis and Purification of Sponge-nanoparticles (miR-181-sponge Nanoparticles)

7:01

Results: Validation of Nanonvector Delivery of the miR-181-sponge and its Effect on miR-181c Expression

7:58

Conclusion

文字起こし

So for the last couple of decades, the importance of microRNA has been recognized in human disease conditions, but the problem is that, therapy-wise, it did not go to the primetime. One of the problem is, one microRNA can have different targets in

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Tissue-specific microRNA inhibition is a technology that is underdeveloped in the microRNA field. Herein, we describe a protocol to successfully inhibit the miR-181 microRNA family in myoblast cells from the heart. Nanovector technology is used to deliver a microRNA sponge that demonstrates significant in vivo cardio-specific miR-181 family inhibition.

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