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Carlsberg Research Laboratory

1 ARTICLES PUBLISHED IN JoVE

Biochemistry

Determination of the Glycogen Content in Cyanobacteria

Alice De Porcellinis¹, Niels-Ulrik Frigaard², Yumiko Sakuragi³

¹Carlsberg Research Laboratory, ²Department of Biology, University of Copenhagen, ³Copenhagen Plant Science Center, Department of Plant and Environmental Sciences, University of Copenhagen

Here, we present a reliable and easy assay to measure the glycogen content in cyanobacterial cells. The procedure entails precipitation, selectable depolymerization, and the detection of glucose residues. This method is suitable for both wildtype and genetically engineered strains and can facilitate the metabolic engineering of cyanobacteria.