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Université de Lyon, ENS de Lyon, UCBL, INRA, CNRS

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Developmental Biology

Use of Atomic Force Microscopy to Measure Mechanical Properties and Turgor Pressure of Plant Cells and Plant Tissues
Simone Bovio 1,2, Yuchen Long 2, Françoise Monéger 2
1SFR Biosciences, Université de Lyon, 2Laboratoire de Reproduction et Développement des Plantes, Université de Lyon, ENS de Lyon, UCBL, INRA, CNRS

Here, we present atomic force microscopy (AFM), operated as a nano- and micro-indentation tool on cells and tissues. The instrument allows the simultaneous acquisition of 3D surface topography of the sample and its mechanical properties, including cell wall Young's modulus as well as turgor pressure.

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