FONDAP Geroscience Center for Brain Health and Metabolism

1 ARTICLES PUBLISHED IN JoVE

Neuroscience

Analyzing the Parkinson's Disease Mouse Model Induced by Adeno-associated Viral Vectors Encoding Human α-Synuclein

Daniela Elgueta¹, Ornella Chovar¹, Valentina Ugalde¹, Vanesa Sánchez-Guajardo¹, Alejandra Catenaccio², Felipe Court^2,3,4, Rodrigo Pacheco^1,5

¹Laboratorio de Neuroinmunología, Fundación Ciencia & Vida, ²Center for Integrative Biology, Universidad Mayor, ³FONDAP Geroscience Center for Brain Health and Metabolism, ⁴Buck Institute for Research on Ageing, ⁵Facultad de Medicina y Ciencia, Universidad San Sebastián

This work analyzes the vector dose and exposure time required to induce neuroinflammation, neurodegeneration, and motor impairment in this preclinical model of Parkinson's disease. These vectors encoding the human α-synuclein are delivered into the substantia nigra to recapitulate the synuclein pathology associated with Parkinson's disease.