Florida Institute of Technology

2 ARTICLES PUBLISHED IN JoVE

Environment

Standardized Methods for Measuring Induction of the Heat Shock Response in Caenorhabditis elegans

Nicole L. Golden¹, Rosemary N. Plagens¹, Karen S. Kim Guisbert¹, Eric Guisbert¹

¹Department of Biomedical and Chemical Engineering and Sciences, Florida Institute of Technology

Here, standardized protocols are presented to assess induction of the heat shock response (HSR) in Caenorhabditis elegans using RT-qPCR at the molecular level, fluorescent reporters at the cellular level, and thermorecovery at the organismal level.

Developmental Biology

In Vivo Visualization of Calcium Transients during Fertilization and Early Development in C. elegans

Michaela Foley¹, Katie Toperzer¹, Sara Sornat¹, Jesse Morgan¹, Savannah Brennan¹, Eric Guisbert¹, Karen Kim Guisbert¹

¹Florida Institute of Technology

Here, we present protocols and tools for visualizing calcium during fertilization and early embryogenesis using a genetically encoded calcium reporter expressed in the germline of the model nematode C. elegans.