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Heartland Center for Mitochondrial Medicine

2 ARTICLES PUBLISHED IN JoVE

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Neuroscience

Use of Capillary Electrophoresis Immunoassay to Search for Potential Biomarkers of Amyotrophic Lateral Sclerosis in Human Platelets
Jessica M. Sage 1, LaSharice Hall 2, April McVey 3, Richard J. Barohn 3, Jeffrey M. Statland 3, Omar Jawdat 3, Mazen M. Dimachkie 3, Abdulbaki Agbas 1
1Department of Basic Sciences, Kansas City University of Medicine and Biosciences, 2Rice University School of Medicine, 3University of Kansas Medical Center

Blood-based biomarkers for neurodegenerative diseases are essential for implementing large-scale clinical studies. A reliable and validated blood test should require a small sample volume as well as be a less invasive sampling method, affordable, and reproducible. This paper demonstrates that high-throughput capillary electrophoresis immunoassay satisfies criteria for potential biomarker development.

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Biochemistry

Measuring Mitochondrial Electron Transfer Complexes in Previously Frozen Cardiac Tissue from the Offspring of Sow: A Model to Assess Exercise-Induced Mitochondrial Bioenergetics Changes
Daniel Barrera 1, Sierra Upton 2, Megan Rauch 3, Tara Notarianni 4, Ki Suk Eum 5, Megan Liberty 6, Sarmila Venkoba Sah 7, Robert Liu 8, Sean Newcomer 9, Linda E. May 10, Emre Agbas 11, Jessica Sage 12, Edina Kosa 7, Abdulbaki Agbas 7,13
1AdventHelath, 2University of Illinois Chicago School of Public Health, 3Lincoln Memorial University, 4Children’s Mercy Hospital, 5Scripps Clinic, 6Cincinnati Children’s Hospital Medical Center, 7Kansas City University, 8Roblex Rex Veterans Affairs Medical Center, 9California State University San Marcos, 10East Carolina University, 11Massachusetts Institute of Technology, 12Boehringer Ingelheim Norway KS, 13Heartland Center for Mitochondrial Medicine

Preparation of mitochondria-enriched samples from previously frozen archived solid tissues allowed the investigators to perform both functional and analytical assessments of mitochondria in various experimental modalities. This study demonstrates how to prepare mitochondria-enriched preparations from frozen heart tissue and perform analytical assessments of mitochondria.

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