JoVE Logo

Sign In

Indiana University Purdue University Indianapolis

1 ARTICLES PUBLISHED IN JoVE

image

Immunology and Infection

Co-culture Models of Pseudomonas aeruginosa Biofilms Grown on Live Human Airway Cells
Sophie Moreau-Marquis 1, Carly V. Redelman 2, Bruce A. Stanton 1, Gregory G. Anderson 2
1Department of Physiology, Dartmouth College, 2Department of Biology, Indiana University Purdue University Indianapolis

This paper describes different methods of growing Pseudomonas aeruginosa biofilms on cultured human airway epithelial cells. These protocols can be adapted to study different aspects of biofilm formation, including visualization of the biofilm, staining of the biofilm, measuring the colony forming units (CFU) of the biofilm, and studying biofilm cytotoxicity.

JoVE Logo

Privacy

Terms of Use

Policies

Research

Education

ABOUT JoVE

Copyright © 2024 MyJoVE Corporation. All rights reserved