Lausanne University Hospital (CHUV) and University of Lausanne

1 ARTICLES PUBLISHED IN JoVE

Neuroscience

Live-imaging of Mitochondrial System in Cultured Astrocytes

Jeanne Espourteille¹, Valentin Zufferey¹, Jean-Honoré Laurent¹, Kevin Richetin¹

¹Department of Psychiatry, Center for Psychiatric Neurosciences, Lausanne University Hospital (CHUV) and University of Lausanne

This article describes the method for mitochondrial time-lapse imaging of astrocyte cultures equipped with MitoTimer biosensor and the resulting multiparametric analysis of mitochondrial dynamics, mobility, morphology, biogenesis, redox state, and turnover.