Oregon Health & Science University, School of Medicine

1 ARTICLES PUBLISHED IN JoVE

Bioengineering

Quantitative Optical Microscopy: Measurement of Cellular Biophysical Features with a Standard Optical Microscope

Kevin G. Phillips^1,2, Sandra M. Baker-Groberg¹, Owen J.T. McCarty^1,3

¹Department of Biomedical Engineering, Oregon Health & Science University, School of Medicine, ²Department of Dermatology, Oregon Health & Science University, School of Medicine, ³Department of Cell & Developmental Biology, Division of Hematology & Medical Oncology, Knight Cancer Institute, Oregon Health & Science University, School of Medicine

We describe the use of a standard optical microscope to perform quantitative measurements of cellular mass, volume, and density through a combination of bright field and differential interference contrast imagery.