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Pacific Northwest Diabetes Research Institute

1 ARTICLES PUBLISHED IN JoVE

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Biology

BEST: Barcode Enabled Sequencing of Tetrads
Adrian C. Scott 1, Catherine L. Ludlow 1, Gareth A. Cromie 1, Aimée M. Dudley 1
1Pacific Northwest Diabetes Research Institute

Barcode Enabled Sequencing of Tetrads (BEST) replaces the manual processes of isolating, disrupting and spacing tetrads. BEST isolates tetrads by fluorescence-activated cell sorting onto agar plates, separates the spores by agitation with glass beads, and determines which randomly arrayed colonies were derived from the same original tetrad using molecular barcodes.

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