Polish-Japanese Academy of Information Technology

1 ARTICLES PUBLISHED IN JoVE

Biochemistry

RIBO-seq in Bacteria: a Sample Collection and Library Preparation Protocol for NGS Sequencing

Natalia Kopik^1,2, Olga Chrobak^1,2, Przemyslaw Latoch^1,3, Mariia Kovalenko^1,2, Agata L. Starosta^1,2

¹Laboratory of Gene Expression, ECOTECH-Complex, Maria Curie-Sklodowska University, ²Department of Molecular Biology, Institute of Biological Sciences, Maria Curie-Sklodowska University, ³Faculty of Information Technology, Polish-Japanese Academy of Information Technology

Here we describe the stages of sample collection and preparation for RIBO-seq in bacteria. Sequencing of the libraries prepared according to these guidelines results in sufficient data for comprehensive bioinformatic analysis. The protocol we present is simple, uses standard laboratory equipment and takes seven days from lysis to obtaining libraries.