Gene Expression Analysis of Endothelial Cells Exposed to Shear Stress Using Multiple Parallel-plate Flow ChambersH.S. Jeffrey Man 1,2, Aravin N. Sukumar 1,2, Kyung Ha Ku 2,3, Michelle K. Dubinsky 1,2, Noeline Subramaniam 1,2, Philip A. Marsden 1,2,3,4
1Institute of Medical Science, University of Toronto, 2Keenan Research Centre in the Li Ka Shing Knowledge Institute, St. Michael's Hospital, 3Department of Laboratory Medicine and Pathobiology, University of Toronto, 4Department of Medicine, University of Toronto
Here, a workflow for the culture and gene expression analysis of endothelial cells under fluid shear stress is presented. Included is a physical arrangement for simultaneously housing and monitoring multiple flow chambers in a controlled environment and the use of an exogenous reference RNA for quantitative PCR.
