Identification of Cyclin-dependent Kinase 1 Specific Phosphorylation Sites by an In Vitro Kinase AssayHeying Cui *1, Kyle M. Loftus *1, Crystal R. Noell 1, Sozanne R. Solmaz 1
1Department of Chemistry, State University of New York at Binghamton
Cyclin-dependent kinase 1 (Cdk1) is activated in the G2 phase of the cell cycle and regulates many cellular pathways. Here, we present a protocol for an in vitro kinase assay with Cdk1, which allows the identification of Cdk1-specific phosphorylation sites for establishing cellular targets of this important kinase.
