Generation and 3-Dimensional Quantitation of Arterial Lesions in Mice Using Optical Projection TomographyNicholas S. Kirkby 1, Lucinda Low 1, Junxi Wu 1, Eileen Miller 1, Jonathan R. Seckl 1, Brian R. Walker 1, David J. Webb 1, Patrick W. F. Hadoke 1
1University/ BHF Centre for Cardiovascular Science, University of Edinburgh, The Queen's Medical Research Institute
Ex vivo analysis of arterial lesions from animal models of cardiovascular disease classically relies on histological and immunohistochemical techniques. These provide 2-dimensional measurements in 3-dimensional lesions. This manuscript describes the generation of arterial lesions for quantitative analysis in 3-dimensions using optical projection tomography.
