Microfluidic Model of Necrotizing Enterocolitis Incorporating Human Neonatal Intestinal Enteroids and a Dysbiotic MicrobiomeLauren C. Frazer 1, Yukihiro Yamaguchi 1, Corey M. Jania 1, Wyatt E. Lanik 2, Qingqing Gong 3, Dhirendra K. Singh 1, Stephen Mackay 1, Natalia S. Akopyants 1, Misty Good 1
1Division of Neonatal-Perinatal Medicine, Department of Pediatrics, University of North Carolina at Chapel Hill, 2University of Nebraska College of Medicine, 3Department of Surgery, Washington University School of Medicine
This protocol describes an in vitro model of necrotizing enterocolitis (NEC), which can be used for mechanistic studies into disease pathogenesis. It features a microfluidic chip seeded with intestinal enteroids derived from the human neonatal intestine, endothelial cells, and the intestinal microbiome of a neonate with severe NEC.
