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University of New Mexico Health Science Center

1 ARTICLES PUBLISHED IN JoVE

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Immunology and Infection

Human Colonoid Monolayers to Study Interactions Between Pathogens, Commensals, and Host Intestinal Epithelium
Julie G. In *1, Jennifer Foulke-Abel *2, Elizabeth Clarke 1, Olga Kovbasnjuk 1
1Department of Internal Medicine, University of New Mexico Health Science Center, 2Department of Medicine, Division of Gastroenterology & Hepatology, Johns Hopkins University School of Medicine

Here, we present a protocol to culture human enteroid or colonoid monolayers that have intact barrier function to study host epithelial-microbiota interactions at the cellular and biochemical level.

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