University of South Florida Morsani College of Medicine

1 ARTICLES PUBLISHED IN JoVE

Medicine

Preparation of Myeloid Derived Suppressor Cells (MDSC) from Naive and Pancreatic Tumor-bearing Mice using Flow Cytometry and Automated Magnetic Activated Cell Sorting (AutoMACS)

Nadine Nelson¹, Karoly Szekeres¹, Denise Cooper¹, Tomar Ghansah¹

¹Department of Molecular Medicine, University of South Florida Morsani College of Medicine

This is a rapid and comprehensive method of immunophenotyping Myeloid Derived Suppressor Cells (MDSC) and enriching Gr-1⁺ leukocytes from mouse spleens. This method uses flow cytometry and AutoMACS Cell Sorting to enrich for viable Gr-1⁺ leukocytes prior to FACS sorting of MDSC for use in vivo and in vitro assays.