Lentivirus Preparation and Reprogramming Transduction for Pancreatic Ductal Adenocarcinoma Cells

1 2 To begin, see the HEK 293T cells3 24 hours before transfection 4 in a 15 centimeter dish containing GMEN. 5 Incubate the cells at 37 degrees Celsius 6 and 5%carbon dioxide 7 until they reach 40 to 50%confluency. 8 Label three 15 milliliter